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rnase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rnase
    Rnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase/Water%2C+RNase%2C+DNase-free/pmc13234486-106-8-13
    Average 99 stars, based on 1 article reviews
    rnase - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Bacillus subtilis pb2441 partially attenuates hepatic steatosis with transcriptomic changes broadly similar to dapagliflozin in a Western diet mouse model.
    Article Snippet: .. The spin column was then placed in a new 1.5 mL collection tube, and RNA was eluted by adding 50 μL of RNase-free water directly to the membrane and centrifuging for 1 minute at 8,000 × g. RNA concentration and purity were assessed using 1 μL of total RNA on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA integrity was evaluated using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and RNA quality was reported as the RNA Integrity Number (RIN).

    Concentration Assay:

    Article Title: Bacillus subtilis pb2441 partially attenuates hepatic steatosis with transcriptomic changes broadly similar to dapagliflozin in a Western diet mouse model.
    Article Snippet: .. The spin column was then placed in a new 1.5 mL collection tube, and RNA was eluted by adding 50 μL of RNase-free water directly to the membrane and centrifuging for 1 minute at 8,000 × g. RNA concentration and purity were assessed using 1 μL of total RNA on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA integrity was evaluated using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and RNA quality was reported as the RNA Integrity Number (RIN).

    Article Title: IGFBP1: A Key Regulatory Gene in the Oncogenesis and Progression of Esophageal Cancer
    Article Snippet: .. Total RNA was resuspended in RNase-free water, and RNA purity and concentration were determined using a NanoDrop spectrophotometer(Thermo Fisher Scientific, Wilmington, NC, USA). .. One microgram (1 μg) of total RNA was reverse transcribed into complementary DNA (cDNA) with the HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (Vazyme Biotech Co., Ltd., Nanjing, China).

    Spectrophotometry:

    Article Title: Bacillus subtilis pb2441 partially attenuates hepatic steatosis with transcriptomic changes broadly similar to dapagliflozin in a Western diet mouse model.
    Article Snippet: .. The spin column was then placed in a new 1.5 mL collection tube, and RNA was eluted by adding 50 μL of RNase-free water directly to the membrane and centrifuging for 1 minute at 8,000 × g. RNA concentration and purity were assessed using 1 μL of total RNA on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA integrity was evaluated using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and RNA quality was reported as the RNA Integrity Number (RIN).

    Article Title: Profiling heterologous 3′ terminator-dependent expression variation in Aspergillus oryzae
    Article Snippet: .. After sequential washing steps, RNA was eluted in RNase-free water, quantified using a NanoDrop spectrophotometer, assessed for purity, and stored at −80 °C until use. cDNA synthesis was performed using 100 ng of total RNA and the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer's instructions. .. Transcript levels of mCherry , eGFP , and the internal reference gene β-tubulin were quantified by TaqMan qPCR using custom oligonucleotide probes (IDT, shown in ) and TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).

    Isolation:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).

    Synthesized:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).

    cDNA Synthesis:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).



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